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General Physiology and Biophysics 2022 General Physiology and Biophysics Vol.41, No.4, p. 329–338, 2022
General Physiology and Biophysics 2022 General Physiology and Biophysics Vol.41, No.4, p. 329–338, 2022
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General Physiology and Biophysics Vol.41, No.4, p. 329–338, 2022 |
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| Title: Arginyl-fructosyl-glucose from red ginseng alleviates TGF-β1-induced epithelial-mesenchymal transition of renal tubular epithelial cells | ||
| Author: Xiangdong Lin, Ming Xiang, Haiying Chenand Xinyu Chen | ||
| Abstract: This study aims to explore the effect and mechanism of arginyl-fructosyl-glucose (AFG) on TGF-β1-induced epithelial-mesenchymal transition (EMT) of renal tubular epithelial cells. HK-2 cells were induced by TGF-β1 and then co-cultured with AFG at different concentrations (0, 25, 50, and 100 μmol/l) for 48 h. The morphology of HK-2 cells was observed under an inverted microscope and the expressions of α-SMA, Vimentin, and E-cadherin were assessed by qRT-PCR, Western blot, and immunofluorescence. The mRNA expressions of ERK and STAT3 were also examined by qRT-PCR, and the protein levels of ERK, STAT3, p-ERK, and p-STAT3 were measured by Western blot. Finally, CCK-8 and transwell assays were used to detect cell proliferation and invasion. TGF-β1 treatment significantly induced EMT in HK-2 cells. The expressions of p-ERK and p-STAT3 were signally increased after TGF-β1 induction, while Mogrol treatment inhibited p-ERK, p-STAT3, α-SMA, and Vimentin expression levels, enhanced E-cadherin expression, and suppressed cell proliferation and invasion. AFG exposure could also inhibit p-ERK, p-STAT3, α-SMA, and Vimentin expressions, promote E-cadherin expression, and markedly inhibit HK-2 cell proliferation and invasion. AFG inhibited TGF-β1-induced EMT of renal tubular epithelial cells by regulating phosphorylation of ERK and STAT3. |
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| Keywords: Arginyl-fructosyl-glucose — Renal tubular epithelial cell — TGF-β1 — ERK — STAT3 — Epithelial-mesenchymal transition | ||
| Published online: 28-Jul-2022 | ||
| Year: 2022, Volume: 41, Issue: 4 | Page From: 329, Page To: 338 | |
| doi:10.4149/gpb_2022026 |
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