General Physiology and Biophysics Vol.26, p.207-213, 2007
|
Title: Effect of the pyridoindole antioxidant stobadine on ATP-utilisation
by renal Na,K-ATPase in rats with streptozotocin-induced diabetes |
Author: N. Vrbjar, S. Strelkova, V. Javorkova, J. Vlkovicova, L. Mezesova, M. Stefek, Z. Kyselova, A. Gajdosikova |
|
Abstract: The effect of the pyridoindole antioxidant stobadine on diabetes-induced changes of Na,KATPase, especially those concerning the utilisation of its substrate ATP, was investigated. Sixteen weeks of streptozotocin-induced diabetes (single i.v. dose of streptozotocin; 55 mg/kg) was followed by decrease in the enzyme activity. This effect was emphasised in the presence of higher concentrations of substrate and in the presence of 8 mmol·l–1 ATP it represented 20%. It might be a consequence of altered functional properties of Na,K-ATPase as suggested by 20% decrease in the Vmax value along with decrease in the Km value by 20%. Administration of 0.05% (w/w) stobadine in the diet to diabetic rats improved the function of renal Na,K-ATPase with respect to utilisation of ATP as suggested by significant increase in the enzyme activity in the whole concentration range of ATP investigated as a consequence of Vmax elevation to the level comparable to absolute controls. In conclusion, stobadine may play a positive role in restoring the functional properties of renal Na,K-ATPase, especially concerning the utilisation of energy derived from hydrolysis of ATP, improving thus the maintenance of ionic homeostasis during diabetes.
|
|
Keywords: Sodium-pump — Kidney — Diabetes — Stobadine |
Year: 2007, Volume: 26, Issue: |
Page From: 207, Page To: 213 |
|
|
|
|
download file |
|
|
|
|