Neoplasma Vol.52, p.517-522, 2005
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Title: The value of dot plot patterns and leukemia-associated phenotypes in AML diagnosis by multiparameter flow cytometry
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Author: T., ZELEZNIKOVA
; O., BABUSIKOVA
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Abstract: The immunophenotypic features in patients with acute myeloid
leukemia (AML) were investigated at diagnosis using
a wide antibody panel including progenitor-associated, myeloid and
lymphoid markers in quadruple combinations. Analyzed
were bone marrow samples from 37 adult and pediatric patients for
exact identification of AML blasts according their
localization on CD45/SSC dot plots and aberrant immunophenotypes
in various subtype of AML.
We found the localization of AML blasts on CD45/SSC dot plots,
which in combination with immunophenotype profile
of blasts allow discrimination of several AML subtypes (M0-M2, M3,
M4/M5 and other types). In 27/37 AML patients
(73%) at least one leukemia-associated phenotype (LAP) was found,
two or more aberrancies coexisted in more than a half
of them (78%). Asynchronous expression was the most frequent type
of LAP (77.8%, 21/27) followed by coexpression of
lymphoid-associated antigens, which occurred in 18/27 (66.7%)
patients.
Presented study showed that leukemic cells of each AML patient had
a unique antigenic profile and could be discriminated
from their normal counterparts based on typical light scatter
profiles and aberrant antigen expression that could further
be used for detection of minimal residual disease.
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Keywords: AML, multiparameter flow cytometry, leukemia-associated aberrant
immunophenotypes
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Year: 2005, Volume: 52, Issue: |
Page From: 517, Page To: 522 |
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