Neoplasma Vol.51, p.368-374, 2004
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Title: Effect of testosterone on growth of P388 leukemia cell line in
vivo and in vitro. Distribution of peripheral blood T lymphocytes
and cell cycle progression
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Author: A., ZAID
; J.P., BUREAU
; S., ABOUDKHIL
; L., HENRY
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Abstract: In transplanted mice, the P388 tumor grew better in castrated than
in non castrated (NC) mice. The proportion of CD8+ in the blood
was more numerous in NC mice. The T cell subsets (CD4+ and CD8+)
were also high in the mice with small tumor tissue (<10 mg). The
correlation observed between the tumor weight and T cell subset in
PBL and in the mice with small tumors could confirm the important
intervention of CD4+ and CD8+ cells to inhibit growth of tumor.
Depo-testosterone (DT) injection reduced strongly weight and tumor
growth in mice and DT administration induced a significant
increase in the percentage of blood CD8+ cells in grafted mice.
The effect of DT was studied on the cell cycle progression, in the
tumor tissue of P388 tumor bearing BDF1 mice and in the P388
murine leukemia cell line in culture. The cell cycle analysis
showed that DT decreased both the cells in S phase and the
proliferating leukemic cells, with accumulation of the cells in
G0/G1phase. The testosterone can inhibit the proliferation of
leukemic cells with a pharmacological dose (10-7M). This growth
inhibition dose and time dependent was associated with cell cycle
arrest; P388 cells accumulates in G0/G1 phase. We also observed a
correlation between tumor weight and the percentage of cells in
G0/G1 and the relative number of cells in proliferative state (S +
G2/M).
Our experiments showed that testosterone prevents the growth of
tumor: indirectly by modulation of subsets T cells distribution
and directly by alteration of the cell cycle.
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Keywords: testosterone, P388 leukemia cell line, peripheral blood
lymphocyte, tumor tissue, subsets T cells, cell cycle
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Year: 2004, Volume: 51, Issue: |
Page From: 368, Page To: 374 |
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