Neoplasma Vol.51, p.38-43, 2004
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Title: Overexpression of cathepsin B correlates with angiogenesis in
colon adenocarcinoma
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Author: W.J., KRUSZEWSKI
; R., RZEPKO
; J., WOJTACKI
; J., SKOKOWSKI
; A., KOPACZ
; K., DRUCIS
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Abstract: Some studies have shown the influence of proteases and vascular
density in colorectal primary tumors on spreading and on the
course of colorectal cancer. In the present study we have analyzed
the relationships between overexpression of cathepsin B protein
and angiogenesis intensity in resected colon tumors and their
impact on prognosis. It has been investigated in a series of 90
colon cancer patients. Immunohistochemistry was used to evaluate
cathepsin B overexpression in cancer cells and to visualize
microvessels with antibodies against von Willebrand factor.
Overexpression of cathepsin B was observed if more than 50% of
cancer cells in searched field showed immunoreactivity with
antibody against cathepsin B. Intensity of angiogenesis was
evaluated as a mean number of microvessels from three fields with
highest vessel number. In 36 cases (40%) overexpression of
cathepsin B was detected. Increased angiogenesis (above median 31
vessels per 0.785 mm2) correlated positively with cathepsin B
overexpression (p=0.0006). Higher vascular density associated with
the presence of metastases in regional lymph nodes (p=0.01).
Overexpression of cathepsin B was observed more often in group of
older people (age above median 65 years; p=0.005). According to
univariate analysis metastases in regional lymph nodes (p=0.0007),
increased angiogenesis (p=0.0085), and distant metastases (p=0.02)
were the features potentially influencing prognosis. Multivariate
analysis revealed independent prognostic value only in case of
metastases in regional lymph nodes (p=0.013) and when distant
metastases were present (p=0.021), but not when increased
angiogenesis in primary colon adenocarcinoma was observed
(p=0.078). In conclusion we can say that there is a close
relationship between intensity of angiogenesis and overexpression
of cathepsin B protein in cancer cells in resected colon
adenocarcinoma.
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Keywords: colon, adenocarcinoma, cathepsin B, angiogenesis, prognosis
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Year: 2004, Volume: 51, Issue: |
Page From: 38, Page To: 43 |
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