General Physiology and Biophysics Vol.26, p.173-180, 2007
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Title: Evaluation of novel microtubules interfering agents myoseverin,
tubulyzine and E2GG in primary cultures of rat hepatocytes |
Author: Z. Dvorak, M. Modriansky, J. Vrba1, J. Ulrichova1, V. Krystof, J. Styskala, P. Pavek |
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Abstract: We investigated the effects of novel microtubules interfering agents (MIAs) in primary cultures of rat hepatocytes. Cells were treated for 24 h with a known compound colchicine and newly synthesized derivatives myoseverin, tubulyzine, and E2GG. We examined the effects of MIAs on microtubules network integrity and on the polymerization capability of isolated tubulin. All tested MIAs inhibited microtubules assembly with the following IC50 values: tubulyzine (4.4 ± 0.9 μmol/l), myoseverin (7.0 ± 0.8 μmol/l), E2GG (16 ± 2 μmol/l), colchicine (2.0 ± 0.4 μmol/l). The potency of MIAs to perturb microtubular network integrity (monitored by immune-histochemistry) increased in the order tubulyzine
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Keywords: Microtubule — Tubulin polymerization — Cytochrome P450 — Cellular signaling
— Myoseverin — Tubulyzine |
Year: 2007, Volume: 26, Issue: |
Page From: 173, Page To: 180 |
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