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Acta Virologica Vol.51, p.51-54, 2007 |
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Title: A NOVEL DOUBLE-STABLE T-REX/GB CELL LINE EXPRESSING GLYCOPROTEIN B OF HERPES SIMPLEX VIRUS 1 | ||
Author: R. TANGELMAYER, V. DURMANOVA, C.W. KNOPF, M. KUDELOVA, I. REZUCHOVA | ||
Abstract: We established and characterized a new stable T-REx/gB cell line expressing gB of HSV-1 under tetracycline (Tet) control. The expression of complete gB (120 K) in T-REx/gB cells was detected by Western blot analysis with anti-HSV-1/ANG/gB monoclonal antibody as early as 2 days after Tet induction. Inducibility and tightness of Tet-regulated gB expression in T-REx/gB cell line was shown to be preserved after long-term culture (2 months) and after numerous freezing/thawing cycles as well. In this study, we described the conditions required for the generation of the T-REx/gB cell line, which can be useful as a host for the isolation and propagation of HSV-1 recombinant viruses defective in gB gene. |
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Keywords: cell line expressing gB; Herpes simplex virus 1; inducible expression | ||
Year: 2007, Volume: 51, Issue: | Page From: 51, Page To: 54 | |
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