Journal info
|
||
Select Journal
Journals
Bratislava Medical Journal Endocrine Regulations General Physiology and Biophysics Neoplasma Acta Virologica Current articles 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudbaWebshop Cart
Your Cart is currently empty.
Acta Virologica Vol.50, p.67-70, 2006 |
||
Title: IN SITU RT-PCR CAN DISTINGUISH BETWEEN PRODUCTIVE AND LATENT CYTOMEGALOVIRUS INFECTION IN THE BLOOD CELLS OF BONE MARROW TRANSPLANT RECIPIENTS | ||
Author: B. ZAWILINSKA, K. BULEK, J. KOPEC, E. DASZKIEWICZ, D. ROJEK-ZAKRZEWSKA, M. KOSZ-VNENCHAK | ||
Abstract: Thirty-four peripheral blood leukocyte samples from bone marrow transplant (BMT) recipients
were examined for Human cytomegalovirus (HCMV) phosphoprotein 65 (pp65), DNA and late transcripts. Twenty seven samples were positive for pp65 in the cytoplasm by immunofluorescent assay (IFA). Viral DNA
was confirmed in 26 samples by nested PCR (nPCR). Using in situ RT-PCR, viral late transcripts were found in 19 samples, positive also by IFA and nPCR; these samples were considered indicative of productive viral
infection. Five samples, positive by nPCR but negative by IFA and in situ RT-PCR, were considered to represent latent viral infection. In 8 samples, positive by IFA and nPCR but negative by in situ RT-PCR, apparently
phagocytosis of viral particles took place. |
||
Keywords: Human cytomegalovirus; in situ RT-PCR; pp65 antigen; viral DNA; viral transcripts; bone marrow transplant patients | ||
Year: 2006, Volume: 50, Issue: | Page From: 67, Page To: 70 | |
|
download file |
|