Neoplasma Vol.50, p.131-138, 2003
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Title: Immunocytochemical detection of p21ras, Raf-1, ERK1/MAP kinase and PKC isoforms in a 20-metylcholantrene-induced transformed murine embryonal fibroblast cells in culture
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Author: K., BANERJEE
; S.K., DAS
; P., MUKHERJEE
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Abstract: An immunocytochemical study using antibodies against p21ras, Raf-1, MAP
kinase/ERK1 and PKCalpha, beta, gamma, delta, epsilon, zeta, isoforms were
performed on a 20-methylcholanthrene-induced transformed murine embryonal
fibroblast cells in both in vitro and in vivo growth conditions. Altered
expression of p21ras, Raf-1, MAP kinase in this particular cell line strongly
supported the previous findings of the activation of one component of signal
transduction under the influence of the other in the MAP kinase cascade of
signal transduction during neoplastic transformation and which also seemed to be
involved in CNCI-PM-20 cell line. The altered expression of PKCalpha, beta, and
delta was thought to be an epigenetic event occurring under the indirect
influence of other changes in these cells. Host physiology and metabolism did
not have much impact on the expression of these gene products after biological
incubation of these cells in syngenic host.
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Keywords: Chemical carcinogenesis, components of MAP kinase cascade, PKC isoforms.
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Year: 2003, Volume: 50, Issue: |
Page From: 131, Page To: 138 |
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