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Acta Virologica Vol.55, No.4, p.295-302, 2011 |
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Title: Rapid identification of YVDD mutants of hepatitis B virus using smart amplification process with competition probe | ||
Author: JIN YANG, JIE LI, GUO-QIANG LOU, LI-JUAN WU, CHEN FENG, NAN-PING WU | ||
Abstract: Accurate and timely detection of drug-associated viral mutants is important during antiviral therapy. Combining Smart Amplification Process (SMAP) with competition probe, an assay specifically designed to detect point mutation at codon 204 of the hepatitis B virus (HBV) polymerase gene was developed. This assay was sensitive to detect 20 copies of mutant/reaction and recognize as little as 1% of minor mutants in the viral population. The comparison of direct sequencing and SMAP method on 35 clinical specimens showed the concordance in 88% of the cases. This method provides an efficient alternative for rapid identification of HBV mutation associated with lamivudine resistance. |
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Keywords: hepatitis B virus; YVDD mutant; SMAP; lamivudine | ||
Year: 2011, Volume: 55, Issue: 4 | Page From: 295, Page To: 302 | |
doi:10.4149/av_2011_04_295 |
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