Neoplasma Vol.55, No.1, p.36-41, 2008
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Title: PCR analysis of immunoglobulin heavy chain and TCR gene
rearrangements in diagnosis of lymphoproliferative disorders
on formalin-fixed, paraffin-embedded tissues |
Author: M. SVACHOVA, M. TICHY |
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Abstract: To confirm a diagnosis of malignant lymphomas it is imperative to distinguish between reactive and neoplastic proliferation. The PCR (polymerase chain reaction) is a method that can be used for detection of clonal rearrangements of the immunoglobulin heavy chain (IgH) and T-cell receptor (TCR) genes. This study summarizes the outcomes of PCR analysis of IgH and TCR gene rearrangements in 91 bioptic cases of lymphoproliferative disorders. In the class of B lymphomas we detected clonal IgH rearrangement in nearly 83% of cases and in class of T lymphomas in 81% of cases. We can affirm that PCR analysis of B and T cell clonality on DNA extracted from the whole section of formalin-fixed, paraffin-embedded tissue is very suitable for routinely elaborate this. Its influence on the diagnostics of morphological unclear cases in particular, is crucial and is useful in establishing a diagnosis of lymphoid neoplasias in specimens in which histological and immunophenotypic studies are inconclusive.
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Keywords: malignant lymphoma, clonality gene rearrangement, polymerase chain reaction |
Year: 2008, Volume: 55, Issue: 1 |
Page From: 36, Page To: 41 |
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