Neoplasma Vol.53, p.155-160, 2006
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Title: Phenotypic quantitative features of patients with acute myeloid leukemia
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Author: F.G., PEREIRA
; K., METZE
; F.P.S., COSTA
; C.S.P., LIMA
; I., LORAND-METZE
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Abstract: The recent WHO classification for acute myeloid leukemias (AML)
separates entities by recurrent cytogenetic abnormalities
and immunophenotypic features presenting prognostic impact. We
have examined the expression of several lineage
and maturation linked antigens used in routine immunophenotyping
of patients with de novo AML, using a 3-color two-step
panel. Cases were diagnosed by peripheral blood counts, bone
marrow cytology, cytochemistry, cytogenetics and
immunophenotyping (CD2, CD3, CD7, CD19, CD13, CD33,
myeloperoxydase – MPO, CD14, CD15, HLA-DR, CD34,
CD56 and CD45). Antigen expression was measured by mean
fluorescence intensity (MFI) by flow cytometry (Paint-a-gate
software). Thirty five patients were analyzed. Median age: 51
years (15–79). Predominant FAB types were M2 and M4. In
6 cases more than one phenotypically distinct blast subpopulation
could be detected. Although our set was small, we tried to
analyze the impact of MFI of the examined antigens on the overall
survival of the patients. In Cox univariate analysis, age,
peripheral leukocytes (WBC) at diagnosis, MFI of CD45, and MPOwere
significant for worse a survival. In the multivariate
analysis only MFI of CD45 and WBC remained in the model (p=0.018
and p=0.014 respectively). After bootstrap
resampling, MFI of CD45 entered the model in 69%,WBCin 60%, age in
42% and MFI ofMPOin 35% of the sets. Analysis
of antigen expression by MFI permitted to detect cases presenting
phenotypically distinct blast subpopulations. This may
represent a pitfall in studies of minimal residual disease by flow
cytometry, as chemotherapy may select one of these
subsets.
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Keywords: acute myeloid leukemia, immunophenotype, flow cytometry, antigen
expression, sub-populations
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Year: 2006, Volume: 53, Issue: |
Page From: 155, Page To: 160 |
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