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Neoplasma Vol.52, p.46-51, 2005 |
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Title: Effect of doxorubicin on actin reorganization in Chinese hamster ovary cells | ||
Author: D., GRZANKA ; J., DOMANIEWSKI ; A., GRZANKA ; | ||
Abstract: Exposure of Chinese hamster ovary (CHO AA8) cells to doxorubicin
in doses of 75 M resulted in reorganization of F-actin filaments
and characteristic feature of apoptosis. Even increase in size of
CHOAA8 cells was observed. Attached cells become more flattered
and elongated than control ones. Intense labeling of F-actin
around the nucleus and disrupted filaments in cytoplasm as well as
stress fibres and bundles of F-actin were seen. Cells detached
were rather rounded and there were not stress fibres present. In
these cells the network of F-actin was weak and rather disrupted
and cells with buds labelled for F-actin were observed. In section
from confocal microscopy labeling of F-actin in nucleus was
confirmed. Electron microscopy showed cells with multisegmented
nuclei. Cells with intracellular area with small and large
vacuoles and containing also electron-dense material were seen.
Other cellular organelles were rather well preserve. There were
margination and condensation of chromatin in nucleus. Immunogold
labelling of actin was observed in cells whether or not treated
with doxorubicin. Positivity for actin was localized in the nuclei
and cytoplasm. In the nucleus gold particles were localized
predominantly in the area of condense chromatin. Positive labeling
for actin was not found after control incubation. These findings
show that doxorubicin promotes changes in the distribution of
actin in CHOAA8 cells and that reorganization of actin in these
cells is involved in process of apoptosis.
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Keywords: CHOAA8 cells, actin, immunogold technique, fluorescence method, doxorubicin | ||
Year: 2005, Volume: 52, Issue: | Page From: 46, Page To: 51 | |
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