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Neoplasma Vol.52, p.52-55, 2005 |
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Title: No evidence of HTLV-I infection in patients with mycosis fungoides and Sezary syndrome | ||
Author: M., PAWLACZYK ; V., FILAS ; M., SOBIESKA ; A., GOZDZICKA-JOZEFIAK ; K., WIKTOROWICZ ; J., BREBOROWICZ ; | ||
Abstract: The involvement of human T-cell lymphotropic virus type I (HTLV-I)
in the etiology of cutaneous T-cell lymphomas (CTCL) is still
controversial. The aim of the study was to evaluate the role of
HTLV-I in the pathogenesis of mycosis fungoides (MF) and Sezary
syndrome (SS) in Polish patients.
The studied group consisted of 42 patients with MF, 5 with SS and
25 with chronic dermatitis. DNA was extracted from snap-frozen and
paraffin-embedded skin biopsies and from peripheral blood.
Polymerase chain reaction (PCR or nested PCR) was carried out for
amplification of different regions of HTLV-I genome. Primer sets
flanking pX, p 19, U5, tax and pol genes were used in the
investigation. The presence of HTLV-I antibody was examined in 46
sera samples with the use of anti-HTLV-I/II EIA test.
HTLV-I antibodies were not detected in any collected sera samples.
PCR with two primer sets homologous to the pX region of HTLV-I
showed negative results in all samples investigated. To confirm
these results two other primer pairs specific for U5 and gag
regions were designed. With these primer pairs no PCR product,
except that in positive control, was observed. For more sensitive
amplification a nested-PCR with pol and tax specific primers was
performed.
HTLV-I probably does not play an important role in the
pathogenesis of MF in Polish patients.
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Keywords: HTLV-I infection, mycosis fungoides, Sezary syndrome | ||
Year: 2005, Volume: 52, Issue: | Page From: 52, Page To: 55 | |
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