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Neoplasma Vol.52, p.36-42, 2005 |
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Title: Application of flow cytometry immunophenotyping and multidrug resistance assay in B-cell acute lymphoid leukemia and multiple myeloma | ||
Author: K., JAKAB ; L., GOPCSA ; E., ADAM ; G., DOMJAN ; B., SARKADI ; K., PALOCZI ; | ||
Abstract: Multidrug resistance is one of the mechanisms how to explain
failure of chemotherapy in patients with different hematological
malignancies. In this study we aimed to evaluate and compare the
drug resistance in B-cell acute lymphoid leukemia (B-ALL) and
multiple myeloma (MM) in association with their immunophenotypes
and genotypes. Eleven patients with B-ALL and 14 patients with MM
were classified according to prognostic factors. Standard MoAb
panel for ALL and triple labeled antibodies (CD38/CD56/CD19) and
detection of intracellular light chains for MM were used. Flow
cytometric calcein assay was performed for measure of P-
glycoprotein (MDR-1) and multidrug resistance associated protein
(MRP-1) activity. Markers CD19, CD20 and HLA-DR proved to be
useful in identifying cells of B-lymphoid lineage. CD34 progenitor
cell antigen was present in high proportion of ALL blasts. Both
the abnormal plasmacell populations and their monoclonality in MM
were confirmed by immunophenotyping, too. The mean MDR activity
factor (MAF) values were not different in patients with MM and B-
ALL. However, the mean MRP-1 values in MM were significantly lower
than MAF-MDR-1 (1.85Ż3.8 versus 5.92Ż7.45, p=0.05), but we have
found lower values in refractory conditions as expected from
previous studies of acute myeloid leukemia. The immunophenotyping
was helpful in detection of abnormal populations showing no
correlation with the MDR. However, in this study we could not
confirm high MDR activity despite of the failure of chemotherapy.
The calcein assay seems to be useful for quantitative and
sensitive measurement of the MDR proteins. The low activity of MDR-
1 and MRP-1 in MM need further clarification, indicating the
involvement of different transport in the resistance mechanism.
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Keywords: multiple myeloma, acute lymphoid leukemia, immunophenotype, intracellular light chain, multidrug resistance | ||
Year: 2005, Volume: 52, Issue: | Page From: 36, Page To: 42 | |
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