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Acta Virologica Vol.49, p.195-205, 2005 |
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Title: COMPLETE NUCLEOTIDE SEQUENCE AND MOLECULAR PROBING OF POTATO VIRUS S GENOME | ||
Author: J. MATOUŠEK, J. SCHUBERT, J. PTÁČEK, P. KOZLOVÁ, P. DĚDIČ | ||
Abstract: Complete genomes of three isolates of Potato virus S (PVS) were cloned and sequenced. The
PVS ORF-1 was characterized for the first time. It encodes a putative replication protein (RPT) that shares the
highest homology (about 52%) with that of Blueberry scorch virus (BlScV). ORF-1 motifs, characteristic for
carlaviruses were found for methyltransferase (MTR), helicase (HEL) and RNA-dependent RNA polymerase
(RdRp). The complete sequence of PVS genome enabled to develop an immunocapture RT-PCR probing of
the PVS genome. Using this system, the sequence variability of 11 genome zones was examined for 34 PVS
isolates including 15 PVS-CS variants that caused a systemic infection in Chenopodium quinoa. A broad
variability between PVS isolates and diverse sequence variants was found. cDNA fragments covering the coat
protein (CP) leader and CP-coding region (approx. 420 bp) were pooled for PVS-O and Chenopodium-systemic
PVS isolates (PVS-CS) and corresponding cDNA libraries were screened for sequence variants. Both cDNA
pools differred mainly in the 5'-end of the CP gene. Methionine at the position 17 in combination with serine
at the position 34 were frequently associated with the CS character of PVS. In general, hydrophobic and polar
amino acids were characteristic for the positions 17 and 34, respectively in PVS-CS isolates. Genome probing
and evolutionary distances suggested that the PVS-CS isolates analyzed were close to the ordinary European
isolates of ordinary strain of PVS (PVS-O) but distant to the original Andean strain of PVS (PVS-A). |
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Keywords: genome sequence; heteroduplex analysis; Potato virus S; RT-PCR; temperature gradient gel electrophoresis; Solanum tuberosum L.; virus genome analysis | ||
Year: 2005, Volume: 49, Issue: | Page From: 195, Page To: 205 | |
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