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General Physiology and Biophysics Vol.39, No.2, 107–122, 2020 |
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Title: Solubilisation of model membrane by DDAO surfactant – partitioning, permeabilisation and liposome-micelle transition | ||
Author: Katarína Želinská, Jana Gallová, Silvia Huláková, Daniela Uhríková, Oleksandr Ivankov | ||
Abstract: Solubilisation of model membranes of dioleoylphosphatidylcholine (DOPC) and DOPC-cholesterol (CHOL) induced by surfactant N,N-dimethyl-1-dodecanamine-N-oxide (DDAO) was studied. At the maintained pH ~ 7.5, the DDAO molecules are in their neutral state with respect to the pK ~ 5. Pore formation in lipid bilayer was studied by fluorescence probe leakage method. The changes in the size of lipid aggregates upon increasing DDAO concentration were followed turbidimetrically. Effective ratio Re at different steps of the solubilisation process was determined. The molar partition coefficient of DDAO in case of the DOPC membrane is Kp = 2262 ± 379, for DOPC-CHOL membrane Kp = 2092 ± 594. Within the experimental error, the partition coefficient, as well as effective ratios Re, are not considerably influenced when one third of DOPC molecules is substituted with CHOL (DOPC:CHOL = 2:1). Constituents of buffer (50 mmol/dm3 PBS, 150 mmol/dm3 NaCl) caused aggregation of DOPC and DOPC-CHOL unilamellar liposomes at zero and low DDAO concentration, as was shown by SANS, turbidimetry and DIC microscopy. After solubilisation of bilayer structures by surfactant, mixed DOPC-DDAO and DOPC-CHOL-DDAO micelles with the shape of cylinders with elliptical cross section were detected. |
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Keywords: N,N-dimethyl-1-dodecanamine-N-oxide — Dioleoylphosphatidylcholine — Cholesterol — Partition coefficient — Solubilisation | ||
Published online: 26-Mar-2020 | ||
Year: 2020, Volume: 39, Issue: 2 | Page From: 107, Page To: 122 | |
doi:10.4149/gpb_2019056 |
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