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Acta Virologica Vol.50, p.53-58, 2006 |
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Title: LYMPHOCYTIC CHORIOMENINGITIS VIRUS MX STRAIN DOES NOT INDUCE THE EXPRESSION OF TUMOR-ASSOCIATED CARBONIC ANHYDRASE IX IN PERSISTENTLY INFECTED HELA CELLS | ||
Author: M. LABUDOVÁ, J. TOMÁŠKOVÁ, M. KALUZOVÁ, J. PASTOREK, S. PASTOREKOVÁ | ||
Abstract: Lymphocytic choriomeningitis virus (LCMV) is an arenavirus that readily causes persistent
infections, in which it does not interfere with vital functions of the cells, but can affect expression of “luxurious” genes. MX strain of LCMV (MX LCMV) has been identified as an agent transmissible by cell-to-cell contact
in the human carcinoma MaTu cells grown in a mixed culture with HeLa cells. When compared to uninfected HeLa, the MaTu-MX-infected HeLa cells, to which the virus was transmitted via co-cultivation with mitomycin C-treated MaTu cells, showed an elevated expression of a protein called MN, suggesting that MN can be induced by MX LCMV. MN protein was later identified as the carbonic anhydrase isoform IX (CA IX), whose
expression has been predominantly associated with hypoxic tumors of poor prognosis. Since the proposal that MX LCMV can induce such a cancer-related protein could substantially change our view on the biology of LCMV-host interaction we undertook its verification. Instead of co-cultivation, we used MaTu cell-free extracts to transmit MX LCMV to HeLa cells. These cells were then grown for more than 30 passages, but the level of MN/CA IX did not increase throughout the whole cultivation period as compared to uninfected HeLa cells. Moreover, a treatment of MaTu-MX-infected HeLa cells with ribavirin eliminated the virus, but did not reduce
the MN/CA IX expression. Our results clearly showed that MN/CA IX is independent of MX LCMV and that the virus itself does not influence the MN/CA IX level in HeLa cells. |
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Keywords: lymphocytic choriomeningitis virus; MX strain; persistent infection; HeLa cells; MaTu cells; carbonic anhydrase IX | ||
Year: 2006, Volume: 50, Issue: | Page From: 53, Page To: 58 | |
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