Journal info
|
||||
Select Journal
Journals
Bratislava Medical Journal 2024 Ahead of print 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 Endocrine Regulations General Physiology and Biophysics Neoplasma Acta Virologica Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudbaWebshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
Bratislava Medical Journal Vol.113, No.5, p.307–310, 2012 |
||
Title: Molecular diagnosis of Toxoplasma gondii in pregnant women | ||
Author: L. Turcekova, F. Spisak, P. Dubinsky, A. Ostro | ||
Abstract: Background: Novel approaches in the diagnostics of T. gondii have enabled a detection of the parasite in the amniotic fluid or blood of pregnant women. Objective: The high titres of IgM and IgG antibodies against T. gondii are not always indicative of the presence of this parasite in pregnant women, therefore the molecular assays can be used to diagnose and genetically characterise T. gondii in amniotic fluids and blood samples. Methods: The study analysed 15 samples of amniotic fluid and 1 sample of the blood from pregnant women suspected for toxoplasmosis. The serological ELISA test was used for the immunological study and molecular analyses, PCR at SAG2 locus followed by RFLP analysis were used for Toxoplasma gondii genotyping. Conclusion: Using PCR assay with TGR1E gene we have confirmed the presence of T. gondii in the blood of a pregnant woman. The parasite was typed as genotype I, belonging to virulent strains (Tab. 1, Fig. 2, Ref. 30). |
||
Keywords: Toxoplasma gondii, molecular methods, PCR analysis, genotype. | ||
Year: 2012, Volume: 113, Issue: 5 | Page From: 307, Page To: 310 | |
doi:10.4149/BLL_2012_071 |
||
|
![]() |
|